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spodoptera frugiperda sf9 cells  (ATCC)


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    ATCC spodoptera frugiperda sf9 cells
    Spodoptera Frugiperda Sf9 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spodoptera+frugiperda/Sf9/10__1038_slash_s41467___026___78235___z-208-10-14
    Average 99 stars, based on 2237 article reviews
    spodoptera frugiperda sf9 cells - by Bioz Stars, 2026-11
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    Related Articles

    Purification:

    Article Title: Insight into the scaffolding function of USP18 from a high resolution cryo-EM structure of STAT2-USP18-ISG15 ternary complex
    Article Snippet: .. SPY-tagged STAT2-USP18-ISG15 complex were co-expressed in Spodoptera frugiperda (Sf9, ATCC CRL-1711) insect cells and purified by tandem affinity purification using Ni-beads (STAT2-USP18) then GST-beads (ISG15). ..

    Article Title: Impact of Influenza Immune Imprinting on Immune Responses to Subsequent Vaccinations in Mice
    Article Snippet: .. GCN4-stabilized trimeric HA3 (from A/Aichi, H3N2), HA1 (from A/Puerto Rico, H1N1), head-removed HA3 (hrHA3), and nucleoprotein (NP) (from A/Puerto Rico, H1N1) were expressed by the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA), and purified with HisPur Ni-NTA Resin (Thermo Scientific, Rockford, IL, USA) from Spodoptera frugiperda (Sf9, ATCC, CRL-1711) cell cultures 11 , 12 . .. Purified proteins were characterized by SDS-PAGE and Western blots (probed with anti-His antibody, Cat. No. ab18184, Abcam).

    Affinity Purification:

    Article Title: Insight into the scaffolding function of USP18 from a high resolution cryo-EM structure of STAT2-USP18-ISG15 ternary complex
    Article Snippet: .. SPY-tagged STAT2-USP18-ISG15 complex were co-expressed in Spodoptera frugiperda (Sf9, ATCC CRL-1711) insect cells and purified by tandem affinity purification using Ni-beads (STAT2-USP18) then GST-beads (ISG15). ..

    Cell Culture:

    Article Title: Influenza Neuraminidase Virus-Like Particle-Based Nanocarriers as a New Platform for the Delivery of Small-Peptide Antigens.
    Article Snippet: A new and simple platform to produce a nanocarrier for small-peptide antigen delivery was developed.. Virus-like particles (VLPs) were of interest due to their good cell-penetrating properties and ability to protect target molecules from degradation.. In this study, the VLP that was entirely formed by influenza neuraminidase (NA), NA-VLPs, was employed.

    Article Title: Structure of the melatonin–related orphan receptor, GPR50
    Article Snippet: These vectors were gifts from Dr Jie-Oh Lee (POSTECH). .. Each baculovirus carrying the constructs was separately amplified in Spodoptera frugiperda (Sf9; CRL-1711, ATCC) insect cells, which were cultured in ESF921 medium (Expression Systems) using the BestBac baculovirus system (Expression Systems). .. The recombinant baculoviruses were cotransfected into Trichoplusia ni HiFive (BTI-TN-5B1-4; Invitrogen) at a density 1.3 × 10 6 cells ml −1 , using a total volume equal to 8% of the medium of HiFive (with heavy chain: light chain volume ratio of 1:1) at 27°C and the medium was collected 72 hours after infection by centrifugation to separate it from the cell pellets.

    Article Title: Structure of the melatonin-related orphan receptor, GPR50.
    Article Snippet: These vectors were gifts from Dr. Jie-Oh Lee (POSTECH). .. Each baculovirus carrying the constructs was separately amplified in Spodoptera frugiperda (Sf9; CRL-1711, ATCC) insect cells, which were cultured in ESF921 medium (Expression Systems) using the BestBac baculovirus system (Expression Systems). .. The recombinant baculoviruses were co-transfected into Trichoplusia ni HiFive (BTI-TN-5B14; Invitrogen) at a density 1.3 × 106 cells ml-1, using a total volume equal to 8 % of the medium of HiFive (with heavy chain : light chain volume ratio of 1:1) at 27 °C and the medium was collected 72 h after infection by centrifugation to separate it from the cell pellets.

    Virus:

    Article Title: Structure basis for the activation of KCNQ2 by endogenous and exogenous ligands.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10Bac Escherichia coli strain Tolobio Cat# CC96136 Chemicals, peptides, and recombinant proteins n-Dodecyl-b-D-Maltopyranoside (DDM) Anatrace Cat# D310 cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 glycol-diosgenin (GDN) Anatrace Cat# GDN101 Tris-base Songen Biotech Cat# A610195-0005 Sodium butyrate Aladdin Cat# S102956 X-treme gene Roche Cat# 6366546001 QO-58 MCE Cat# HY-110162 PI(4,5)P2 DIC8 Echelon Biosciences Cat# P-4508 PI(4,5)P2 Sigma Cat# 850185P TEMED OriLeaf Cat# W10471 amphotericin B Sigma Cat# A4888 PrimeSTAR® HS (Premix) Takara Cat# R040A QuickCut Restriction Enzyme Takara Cat# 1623 DNA Ligation Kit Ver.2.1(solution 1) Takara Cat# 6022 DNA marker Tsingke Cat# TSJ105-500 Critical commercial assays Superose 6, 10/300 GL Cytiva Cat# GE29-0915-96 Deposited data Coordinates of KCNQ2-CaM QO-58 This paper PDB: 9L8W Cryo-EM map of KCNQ2-CaM QO-58 This paper EMDB: EMD-62892 Coordinates of KCNQ2-CaM QO-58-PIP2 This paper PDB: 9L97 Cryo-EM map of KCNQ2-CaM QO-58-PIP2 This paper EMDB: EMD-62896 Coordinates of KCNQ2-CaM QO-83 This paper PDB: 8XO1 Cryo-EM map of KCNQ2-CaM QO-83 This paper EMDB: EMD-38522 Coordinates of KCNQ2-CaM QO-83-PIP2 This paper PDB: 9L9N Cryo-EM map of KCNQ2-CaM QO-83-PIP2 This paper EMDB: EMD-62910 Experimental models: Cell lines Human Embryonic Kidney HEK293S-GnTi cell line ATCC CRL-3022 Human Embryonic Kidney HEK293T cell line ATCC CRL-11268 Spodoptera frugiperda Sf9 cells ATCC CRL-1711 Hamster: CHO cells ATCC CRL-11268 CHO cells stably expressing KCNQ2 channels Laboratory of Professor Qingzhong Jia from Hebei Medical University N/A CHO cells stably expressing KCNQ2 channels Laboratory of Professor Qingzhong Jia from Hebei Medical University N/A HEK293 cells stably expressing KCNQ1/ KCNE1 channels Laboratory of Professor Qingzhong Jia from Hebei Medical University N/A Recombinant DNA Plasmid: pcDNA3.1-KCNQ2-T2A-EGFP This paper N/A (Continued on next page) 14 Cell Reports 45, 116771, January 27, 2026 .. Spodoptera frugiperda (Sf9, ATCC) cells for virus production were maintained in SIM SF expression medium (Sino Biological Inc.) at 27 ◦ C with gentle shaking at 120 rpm. .. HEK293S (ATCC) cells for protein expression were maintained in SMM 293-TI complete medium (Sino Biological Inc.) at 37 ◦ C with gentle shaking at 130 rpm.

    Expressing:

    Article Title: Structure basis for the activation of KCNQ2 by endogenous and exogenous ligands.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10Bac Escherichia coli strain Tolobio Cat# CC96136 Chemicals, peptides, and recombinant proteins n-Dodecyl-b-D-Maltopyranoside (DDM) Anatrace Cat# D310 cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 glycol-diosgenin (GDN) Anatrace Cat# GDN101 Tris-base Songen Biotech Cat# A610195-0005 Sodium butyrate Aladdin Cat# S102956 X-treme gene Roche Cat# 6366546001 QO-58 MCE Cat# HY-110162 PI(4,5)P2 DIC8 Echelon Biosciences Cat# P-4508 PI(4,5)P2 Sigma Cat# 850185P TEMED OriLeaf Cat# W10471 amphotericin B Sigma Cat# A4888 PrimeSTAR® HS (Premix) Takara Cat# R040A QuickCut Restriction Enzyme Takara Cat# 1623 DNA Ligation Kit Ver.2.1(solution 1) Takara Cat# 6022 DNA marker Tsingke Cat# TSJ105-500 Critical commercial assays Superose 6, 10/300 GL Cytiva Cat# GE29-0915-96 Deposited data Coordinates of KCNQ2-CaM QO-58 This paper PDB: 9L8W Cryo-EM map of KCNQ2-CaM QO-58 This paper EMDB: EMD-62892 Coordinates of KCNQ2-CaM QO-58-PIP2 This paper PDB: 9L97 Cryo-EM map of KCNQ2-CaM QO-58-PIP2 This paper EMDB: EMD-62896 Coordinates of KCNQ2-CaM QO-83 This paper PDB: 8XO1 Cryo-EM map of KCNQ2-CaM QO-83 This paper EMDB: EMD-38522 Coordinates of KCNQ2-CaM QO-83-PIP2 This paper PDB: 9L9N Cryo-EM map of KCNQ2-CaM QO-83-PIP2 This paper EMDB: EMD-62910 Experimental models: Cell lines Human Embryonic Kidney HEK293S-GnTi cell line ATCC CRL-3022 Human Embryonic Kidney HEK293T cell line ATCC CRL-11268 Spodoptera frugiperda Sf9 cells ATCC CRL-1711 Hamster: CHO cells ATCC CRL-11268 CHO cells stably expressing KCNQ2 channels Laboratory of Professor Qingzhong Jia from Hebei Medical University N/A CHO cells stably expressing KCNQ2 channels Laboratory of Professor Qingzhong Jia from Hebei Medical University N/A HEK293 cells stably expressing KCNQ1/ KCNE1 channels Laboratory of Professor Qingzhong Jia from Hebei Medical University N/A Recombinant DNA Plasmid: pcDNA3.1-KCNQ2-T2A-EGFP This paper N/A (Continued on next page) 14 Cell Reports 45, 116771, January 27, 2026 .. Spodoptera frugiperda (Sf9, ATCC) cells for virus production were maintained in SIM SF expression medium (Sino Biological Inc.) at 27 ◦ C with gentle shaking at 120 rpm. .. HEK293S (ATCC) cells for protein expression were maintained in SMM 293-TI complete medium (Sino Biological Inc.) at 37 ◦ C with gentle shaking at 130 rpm.

    Gentle:

    Article Title: Structure basis for the activation of KCNQ2 by endogenous and exogenous ligands.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains DH10Bac Escherichia coli strain Tolobio Cat# CC96136 Chemicals, peptides, and recombinant proteins n-Dodecyl-b-D-Maltopyranoside (DDM) Anatrace Cat# D310 cholesteryl hemisuccinate (CHS) Anatrace Cat# CH210 glycol-diosgenin (GDN) Anatrace Cat# GDN101 Tris-base Songen Biotech Cat# A610195-0005 Sodium butyrate Aladdin Cat# S102956 X-treme gene Roche Cat# 6366546001 QO-58 MCE Cat# HY-110162 PI(4,5)P2 DIC8 Echelon Biosciences Cat# P-4508 PI(4,5)P2 Sigma Cat# 850185P TEMED OriLeaf Cat# W10471 amphotericin B Sigma Cat# A4888 PrimeSTAR® HS (Premix) Takara Cat# R040A QuickCut Restriction Enzyme Takara Cat# 1623 DNA Ligation Kit Ver.2.1(solution 1) Takara Cat# 6022 DNA marker Tsingke Cat# TSJ105-500 Critical commercial assays Superose 6, 10/300 GL Cytiva Cat# GE29-0915-96 Deposited data Coordinates of KCNQ2-CaM QO-58 This paper PDB: 9L8W Cryo-EM map of KCNQ2-CaM QO-58 This paper EMDB: EMD-62892 Coordinates of KCNQ2-CaM QO-58-PIP2 This paper PDB: 9L97 Cryo-EM map of KCNQ2-CaM QO-58-PIP2 This paper EMDB: EMD-62896 Coordinates of KCNQ2-CaM QO-83 This paper PDB: 8XO1 Cryo-EM map of KCNQ2-CaM QO-83 This paper EMDB: EMD-38522 Coordinates of KCNQ2-CaM QO-83-PIP2 This paper PDB: 9L9N Cryo-EM map of KCNQ2-CaM QO-83-PIP2 This paper EMDB: EMD-62910 Experimental models: Cell lines Human Embryonic Kidney HEK293S-GnTi cell line ATCC CRL-3022 Human Embryonic Kidney HEK293T cell line ATCC CRL-11268 Spodoptera frugiperda Sf9 cells ATCC CRL-1711 Hamster: CHO cells ATCC CRL-11268 CHO cells stably expressing KCNQ2 channels Laboratory of Professor Qingzhong Jia from Hebei Medical University N/A CHO cells stably expressing KCNQ2 channels Laboratory of Professor Qingzhong Jia from Hebei Medical University N/A HEK293 cells stably expressing KCNQ1/ KCNE1 channels Laboratory of Professor Qingzhong Jia from Hebei Medical University N/A Recombinant DNA Plasmid: pcDNA3.1-KCNQ2-T2A-EGFP This paper N/A (Continued on next page) 14 Cell Reports 45, 116771, January 27, 2026 .. Spodoptera frugiperda (Sf9, ATCC) cells for virus production were maintained in SIM SF expression medium (Sino Biological Inc.) at 27 ◦ C with gentle shaking at 120 rpm. .. HEK293S (ATCC) cells for protein expression were maintained in SMM 293-TI complete medium (Sino Biological Inc.) at 37 ◦ C with gentle shaking at 130 rpm.

    Construct:

    Article Title: Structure of the melatonin–related orphan receptor, GPR50
    Article Snippet: These vectors were gifts from Dr Jie-Oh Lee (POSTECH). .. Each baculovirus carrying the constructs was separately amplified in Spodoptera frugiperda (Sf9; CRL-1711, ATCC) insect cells, which were cultured in ESF921 medium (Expression Systems) using the BestBac baculovirus system (Expression Systems). .. The recombinant baculoviruses were cotransfected into Trichoplusia ni HiFive (BTI-TN-5B1-4; Invitrogen) at a density 1.3 × 10 6 cells ml −1 , using a total volume equal to 8% of the medium of HiFive (with heavy chain: light chain volume ratio of 1:1) at 27°C and the medium was collected 72 hours after infection by centrifugation to separate it from the cell pellets.

    Article Title: Structure of the melatonin-related orphan receptor, GPR50.
    Article Snippet: These vectors were gifts from Dr. Jie-Oh Lee (POSTECH). .. Each baculovirus carrying the constructs was separately amplified in Spodoptera frugiperda (Sf9; CRL-1711, ATCC) insect cells, which were cultured in ESF921 medium (Expression Systems) using the BestBac baculovirus system (Expression Systems). .. The recombinant baculoviruses were co-transfected into Trichoplusia ni HiFive (BTI-TN-5B14; Invitrogen) at a density 1.3 × 106 cells ml-1, using a total volume equal to 8 % of the medium of HiFive (with heavy chain : light chain volume ratio of 1:1) at 27 °C and the medium was collected 72 h after infection by centrifugation to separate it from the cell pellets.

    Amplification:

    Article Title: Structure of the melatonin–related orphan receptor, GPR50
    Article Snippet: These vectors were gifts from Dr Jie-Oh Lee (POSTECH). .. Each baculovirus carrying the constructs was separately amplified in Spodoptera frugiperda (Sf9; CRL-1711, ATCC) insect cells, which were cultured in ESF921 medium (Expression Systems) using the BestBac baculovirus system (Expression Systems). .. The recombinant baculoviruses were cotransfected into Trichoplusia ni HiFive (BTI-TN-5B1-4; Invitrogen) at a density 1.3 × 10 6 cells ml −1 , using a total volume equal to 8% of the medium of HiFive (with heavy chain: light chain volume ratio of 1:1) at 27°C and the medium was collected 72 hours after infection by centrifugation to separate it from the cell pellets.

    Article Title: Structure of the melatonin-related orphan receptor, GPR50.
    Article Snippet: These vectors were gifts from Dr. Jie-Oh Lee (POSTECH). .. Each baculovirus carrying the constructs was separately amplified in Spodoptera frugiperda (Sf9; CRL-1711, ATCC) insect cells, which were cultured in ESF921 medium (Expression Systems) using the BestBac baculovirus system (Expression Systems). .. The recombinant baculoviruses were co-transfected into Trichoplusia ni HiFive (BTI-TN-5B14; Invitrogen) at a density 1.3 × 106 cells ml-1, using a total volume equal to 8 % of the medium of HiFive (with heavy chain : light chain volume ratio of 1:1) at 27 °C and the medium was collected 72 h after infection by centrifugation to separate it from the cell pellets.

    Suspension:

    Article Title: Direct interaction between RSV polymerase L and active Rab11a mediates viral ribonucleoprotein transport to assembly sites
    Article Snippet: .. Spodoptera frugiperda (SF9; ATCC, CRL-1711 Gibco cat# 11496015) were propagated in suspension using Sf-900 II serum-free medium (Gibco cat# 10902096) at 28°C without CO 2 . ..



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    IRBIT binding to PLCδ 1 -PH depends on its serine-rich region A. Surface plasmon resonance (SPR) measurements of GST/PLCδ 1 -PH binding to immobilized full-length His 6 -tagged IRBIT produced in Sf9 cells. Wild-type IRBIT (IRBIT-WT; black) is compared with variants in which the seven serines highlighted in yellow and numbered in the IRBIT N-terminal sequence fragment shown at the top of the figure were replaced by alanines (IRBIT-A 7 ; gray) or glutamates (IRBIT-E 7 ; green). Failure to phosphorylate these serines also prevents binding. Responses were corrected by subtraction of a mock-coupled surface and expressed as a percentage of the maximal SPR response. These experiments are representative of at least three repeats. B. Biolayer interferometry (BLI) concentration/response measurements for soluble full-length IRBIT variants binding to GST/PLCδ 1 -PH immobilized on Octet anti-GST biosensors. IRBIT-WT (black), S66A/S70A/S77A-mutated IRBIT (magenta), S68A/S71A/S74A-mutated IRBIT (olive), and IRBIT-A 7 (gray) are compared. Immobilized GST/Dyn1-PH (maroon) provides a low-binding control. Individual points show the mean response during the final 20 s of association in the BLI experiment, after subtraction of the corresponding response for equivalent sensors on which GST-only had been immobilized. Responses were normalized to the mean fitted B max for IRBIT-WT and IRBIT S66A/S70A/S77A in the same experiment. The data shown are representative of three independent experiments. C. BLI association traces for binding of IRBIT-WT from a 5 μM solution to sensor-immobilized GST/PLCδ 1 -PH with no soluble competitor (in matched buffer; black/gray) or in the presence of 25 μM Ins(1,4,5) P 3 (red) as competitor. Traces were aligned at association onset, corrected by subtraction of signal from a matched GST-only sensor (and competitor-only signals), and normalized to the endpoint obtained with no competitor Fainter lines represent individual experiments; bold lines show means (no competitor, n = 4; Ins(1,4,5) P 3 , n = 3). One sensor was used per condition within each experiment.

    Journal: bioRxiv

    Article Title: Recognition of negative charge arrays by pleckstrin homology (PH) domains

    doi: 10.64898/2026.09.21.753330

    Figure Lengend Snippet: IRBIT binding to PLCδ 1 -PH depends on its serine-rich region A. Surface plasmon resonance (SPR) measurements of GST/PLCδ 1 -PH binding to immobilized full-length His 6 -tagged IRBIT produced in Sf9 cells. Wild-type IRBIT (IRBIT-WT; black) is compared with variants in which the seven serines highlighted in yellow and numbered in the IRBIT N-terminal sequence fragment shown at the top of the figure were replaced by alanines (IRBIT-A 7 ; gray) or glutamates (IRBIT-E 7 ; green). Failure to phosphorylate these serines also prevents binding. Responses were corrected by subtraction of a mock-coupled surface and expressed as a percentage of the maximal SPR response. These experiments are representative of at least three repeats. B. Biolayer interferometry (BLI) concentration/response measurements for soluble full-length IRBIT variants binding to GST/PLCδ 1 -PH immobilized on Octet anti-GST biosensors. IRBIT-WT (black), S66A/S70A/S77A-mutated IRBIT (magenta), S68A/S71A/S74A-mutated IRBIT (olive), and IRBIT-A 7 (gray) are compared. Immobilized GST/Dyn1-PH (maroon) provides a low-binding control. Individual points show the mean response during the final 20 s of association in the BLI experiment, after subtraction of the corresponding response for equivalent sensors on which GST-only had been immobilized. Responses were normalized to the mean fitted B max for IRBIT-WT and IRBIT S66A/S70A/S77A in the same experiment. The data shown are representative of three independent experiments. C. BLI association traces for binding of IRBIT-WT from a 5 μM solution to sensor-immobilized GST/PLCδ 1 -PH with no soluble competitor (in matched buffer; black/gray) or in the presence of 25 μM Ins(1,4,5) P 3 (red) as competitor. Traces were aligned at association onset, corrected by subtraction of signal from a matched GST-only sensor (and competitor-only signals), and normalized to the endpoint obtained with no competitor Fainter lines represent individual experiments; bold lines show means (no competitor, n = 4; Ins(1,4,5) P 3 , n = 3). One sensor was used per condition within each experiment.

    Article Snippet: Spodoptera frugiperda Sf9 cells (Expression Systems), originally established from immature ovaries of female S. frugiperda pupae, were propagated at 27°C with constant shaking at 120 rpm in either serum-free ESF 921 Insect Cell Culture Medium (Expression Systems) containing 50 U/mL penicillin/streptomycin or Insect-XPRESS medium (Lonza), and were used for production of all proteins.

    Techniques: Binding Assay, SPR Assay, Produced, Sequencing, Concentration Assay, Control